ChemoMetec NC-200 NucleoCounter Automated Cell Counter

The ChemoMetec NC-200 NucleoCounter is a high-precision benchtop automated cell counter designed for mammalian cell culture, bioprocessing, and cell therapy workflows. Operating on advanced fluorescence image cytometry, the system delivers objective, user-independent cell counts and viability assessments in under 50 seconds. Paired with a dedicated laptop running NucleoView software, it provides an integrated, plug-and-play analytical workstation.

ChemoMetec NC-200 Measurement and Optical Performance

The system eliminates operator subjectivity through automated fluorophore detection and calibrated consumables:

• Dual Fluorescence Detection: The optical engine uses dual LED excitation sources (365 nm and 505 nm) coupled with a dual-band emission filter. Consequently, it accurately identifies Acridine Orange and DAPI fluorescence to distinguish viable from non-viable nucleated cells.
• Via1-Cassette Technology: The system utilizes volume-calibrated disposable Via1-Cassettes pre-loaded with fluorescent dyes. Therefore, operators avoid manual pipetting, toxic dye handling, and hemocytometer calibration errors.
• Aggregated and Clumpy Cell Analysis: Specialized algorithms dissect clumped samples and cells grown on microcarriers. As a result, it determines accurate counts, viability percentages, mean cell diameter, and aggregation rates without enzymatic pre-digestion.
• Fast Sample Throughput: Standard one-step viability protocols complete in approximately 50 seconds, accelerating sample processing across high-volume production queues.

ChemoMetec NC-200 Hardware and Software Integration

Designed for research and regulated manufacturing suites, the benchtop unit requires minimal footprint and zero routine fluidic cleaning:

• Fluidics-Free Architecture: Because all sample loading and staining occur entirely within the disposable cassette, the internal instrument components remain free of biological fluids. Thus, the system eliminates cross-contamination risks and fluid line clogging.
• Dedicated Laptop Station: The accompanying pre-configured laptop runs NucleoView NC-200 software, providing visual verification of counted cells, customizable gating, and direct protocol selection.
• Regulatory Compliance: The platform is 21 CFR Part 11 and GMP ready, featuring automated audit trails, user access controls, and electronic sign-offs.
• Comprehensive Data Export: Test records export automatically to PDF, CSV, and Excel formats, simplifying documentation for quality assurance teams.

Laboratory Applications and Industry Uses

The instrument serves diverse cell culture environments, including monoclonal antibody production, CAR-T immunotherapy manufacturing, stem cell expansion, and vaccine formulation. In addition, its robust optical linearity supports monitoring from low seeding densities up to 1 × 10⁷ cells/mL.  

Ultimately, the ChemoMetec NC-200 NucleoCounter workstation equips biotechnology laboratories with reliable, audit-ready automated cell enumeration without ongoing maintenance overhead.